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KMID : 0366119950230050531
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Korean Journal of Applied Microbiology & Bioengineering
1995 Volume.23 No. 5 p.531 ~ p.534
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Production of Eicosapentaenoic Acid by Pseudomonas sp. CH-414
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Kim Chang-Ho
Hong Eock-Kee
Shin Won-Cheol
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Abstract
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The identification of eicosapentaenoic acid (EPA) by Pseudomonas sp. CH-414 were investigated under the optimal culture conditions. The maximum dry cell weight and phospholipid production showed about 10 mg/ml and 0.6 mg/ml, respectively, at the 48 hr cultivation. The phospholipid form produced by Pseudomonas sp. CH-414 was elucidated as a phosphatidyt ethanolamine by thin layer chromatography. EPA was contained about 15% in the. extractable lipid, and the amount of EPA was about 83 μg/ml from the culture broth. Also myristic, palmitic, palmitoleic, stearic and oleic acids were identified with gas chromatography.
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KEYWORD
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Eicosapentaenoic acid, Pseudomonas sp. CH-414
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